ON THE EXPRESSION PROFILE OF CANDIDATE GENES CONFERRING RESISTANCE TO GASTRO-INTESTINAL NEMATODES IN SHEEP


Shiv Hiriyur-Nagaraj, Antonio Reverter, Moira Menzies, Nick Andronicos and Aaron Ingham
CSIRO Livestock Industries, 306 Carmody Road, St. Lucia, Qld 4067, Australia

SUMMARY

We report on the integration of a number of RT-PCR expression datasets totalling 102 genes across 49 experimental conditions of relevance to gastro-intestinal nematodes in sheep. The experimental conditions include three challenge trials, six tissues, five genotypes and two parasites surveyed at multiple time points. After mining gene interaction data for these 102 genes, we have generated an interaction network that enabled us to study these genes in a biologically relevant, system context. Following a systematic investigation of this network, we have identified a number of genes encoding extracellular matrix proteins that may be useful biomarkers enabling identification of parasite resistant sheep.

 

Figure 1: The PermutMatrix file containing the data for 49 experimental conditions and 102 genes can be found here and the image generated by hierarchal clustering can be found here. The text file can be opened using PermutMatrix software.

 

Figure 2

 

The network file used to generate Figure 2 is available here for download and can be opened using cytoscape.

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